The diversity of free amino acids in 14 representative vietnamese rice accessions
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https://doi.org/10.15625/2615-9023/22372Keywords:
amino acids, chromatography, diversity, Oryza sativa LAbstract
Rice (Oryza sativa L.) serves as the primary dietary staple for approximately half of the global population. Amino acids play a critical role in plant biology as they constitute the fundamental components of proteins. The free amino acid (FAA) content in rice holds nutritional significance due to its observable influence on the sensory qualities of cooked rice. In this study, 14 rice accessions from diverse ecosystems in Vietnam were selected for analysis of their FAA profiles to identify rice accessions possessing elevated levels of valuable FAA. High-performance liquid chromatography using the Biochrom 30+ system was employed for this purpose. The findings revealed substantial variability in FAA content among the rice accessions evaluated. Notably, the genotype G52 (BA TRANG HUONG) exhibited the highest concentration of FAA, indicating its potential utility as a genetic resource in breeding programs. Essential amino acids constituted approximately 36.1% of the total FAA content. Furthermore, significant differences in FAA, essential amino acids (EAA), and non-essential amino acids (NEAA) were observed between Indica and Japonica genotypes. Among the five amino acid families assessed, the glutamate and aspartate families demonstrated the highest concentrations. The outcomes of this study provide valuable insights that could inform future breeding efforts aimed at enhancing the FAA profile of rice varieties within the Vietnamese rice collection.
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References
Amankwah E. N., 2015. Amino acid profiles of some varieties of rice, soybean and groundnut grown in Ghana. J. Food. Process. Technol., 06: 420. https://doi.org/ 10.4172/2157-7110.1000420
Amrinola W., Sitanggang A. B., Kusnandar F., Budijanto S., 2022. Characterization of pigmented and non-pigmented flakes glutinous rice (ampiang) on chemical compositions, free fatty acids compositions, amino acids compositions, dietary fiber content, and antioxidant properties. Food. Sci. Technol., 42: e86621. https://doi.org/10.1590/FST.86621
Awan T. H., Ahmadizadeh M., Jabran K., Hashim S., Chauhan B. S., 2017. Domestication and development of rice cultivars. In: Chauhan, B., Jabran, K., Mahajan, G. (eds) Rice Production Worldwide. Springer, Cham: 207-216. https://doi.org/10.1007/978-3-319-47516-5_9
Braspaiboon S., Osiriphun S., Peepathum P., Jirarattanarangsri W., 2020. Comparison of the effectiveness of alkaline and enzymatic extraction and the solubility of proteins extracted from carbohydrate-digested rice. Heliyon., 6: e05403. https://doi: 10.1016/j.heliyon.2020.e05403
Chaudhari P. R., Tamrakar N., Singh L., Tandon A., Sharma D., 2018. Rice nutritional and medicinal properties: A review article. J. Pharmacogn. Phytochem., 7: 150–156.
Culea M., Scrob S., Suvar S., Tandon A., Sharma D., 2015. Determination of amino acids in corn seed by gas chromatography–mass spectrometry. Anal. Lett., 48: 37–46. https://doi.org/10.1080/00032719.2014.930869
Dajanta K., Apichartsrangkoon A., Chukeatirote E., Frazier R. A., 2011. Free-amino acid profiles of thua nao, a Thai fermented soybean. Food. Chem., 125: 342–347. https://doi.org/10.1016/J.FOOD CHEM.2010.09.002
Galili G., Amir R., Fernie A. R., 2016. The regulation of essential amino acid synthesis and accumulation in plants. Annu. Rev. Plant. Biol., 67: 153–178. https://doi.org/10.1146/ANNUREV-ARPLANT-043015-112213
Horovitz O., Paşca R. D., 2017. Classification of amino acids by multivariate data analysis, based on thermodynamic and structural characteristics. Stud. Univ. Babes-Bolyai. Chem. 62: 19–31. https://doi.org/10.24193/SUBBCHEM.2017.2.02
Hou Y., Yin Y., Wu G., 2015. Dietary essentiality of “nutritionally non-essential amino acids” for animals and humans. Exp. Biol. Med., 240: 997. https://doi.org/ 10.1177/1535370215587913
Hu C., Shi J., Quan S., Cui B., Kleessen S., Nikoloski Z., Tohge T., Alexander D., Guo L., Lin H., Wang J., Cui X., Rao J., Luo Q., Zhao X., Fernie A. R., Zhang D., 2014. Metabolic variation between japonica and indica rice cultivars as revealed by non-targeted metabolomics. Sci. Reports., 41(4): 1–10. https://doi.org/ 10.1038/srep05067
Huang M., Zhang H., Zhao C., Guanghui C., Yingbin Z., 2019. Amino acid content in rice grains is affected by high temperature during the early grain-filling period. Sci. Reports, 91(9): 1–7. https://doi.org/ 10.1038/s41598-019-38883-2
Ikehashi H., 2009. Why are There indica type and japonica type in rice? - history of the studies and a view for origin of two types. Rice Sci., 16: 1–13. https://doi.org/ 10.1016/S1672-6308(08)60050-5
Kamara J. S., Konishi S., Sasanuma T., Abe T., 2010. Variation in free amino acid profile among some rice (Oryza sativa L.) cultivars. Breed Sci. 60: 46–54. https://doi.org/10.1270/JSBBS.60.46
Kasumyan A.O., 2016. Taste attractiveness of free amino acids and their physicochemical and biological properties (as exemplified by fishes). J. Evol. Biochem. Physiol., 52: 271–281. https://doi.org/10.1134/S0022093016040013
Kawai M., Uneyama H., Miyano H., 2009. Taste-active components in foods, with concentration on umami compounds. J. Heal. Sci., 55: 667–673. https://doi.org/ 10.1248/JHS.55.667
Kowalska S., Szłyk E., Jastrzębska A., 2022. Simple extraction procedure for free amino acids determination in selected gluten-free flour samples. Eur. Food Res. Technol., 248: 507–517. https://doi.org/ 10.1007/S00217-021-03896-7
Mahanama R., Somasiri S., Punyasiri N., Kottawa-Arachchi J. D., 2020. Total and free amino acid contents of popular rice varieties (Oryza sativa L.) consumed in the capital city of Sri Lanka. J. Natl. Sci. Found Sri Lanka, 48: 199–211. https://doi.org/10.4038/jnsfsr.v48i2.9565
Martin M., Fitzgerald M. A., 2002. Proteins in rice grains influence cooking properties! J. Cereal Sci., 36: 285–294. https://doi.org/10.1006/JCRS.2001.0465
Mishra M., Rathore R. S., Singla-Pareek S. L., Pareek A., 2022. High lysine and high protein-containing salinity-tolerant rice grains (Oryza sativa cv IR64). Food Energy Secur., 11: e343. https://doi.org/ 10.1002/FES3.343
Nagaoka H., 2005. Treatment of germinated wheat to increase levels of GABA and IP6 catalyzed by endogenous enzymes. Biotechnol. Prog. 21: 405–410. https://doi.org/10.1021/BP0496777
Nguyen H. C., Hoefgen R., Hesse H., 2012. Improving the nutritive value of rice seeds: elevation of cysteine and methionine contents in rice plants by
ectopic expression of a bacterial serine acetyltransfera. J. Exp. Bot., 63: 5991–6001
Nguyen V. P., Le T. V. A., To H. T. M., Nguyen T. K. O., Mai T. P. N., 2023. Systemic adaptation of rice plants under low phosphate conditions and interaction with endophytic bacteria. Ital. J. Agron., 18. https://doi.org/10.4081/IJA.2023.2181
Rego A., Mota C., Gueifão S., Ventura M., Delgado I., Lopes J., Matos A., Castanheiraet I., 2018. Amino acid contents and toxically relevant arsenic of rice varieties consumed in Portugal. Measurement, 113: 189–195. https://doi.org/10.1016/J.MEASUREMENT.2017.03.025
Wong K. H., Abdul Aziz S., Mohamed S., 2008. Sensory aroma from Maillard reaction of individual and combinations of amino acids with glucose in acidic conditions. Int. J. Food Sci. Technol., 43: 1512–1519. https://doi.org/10.1111/ J.1365-2621.2006.01445.X
Wu G., 2009. Amino acids: metabolism, functions, and nutrition. Amin Acids, 371(37): 1–17. https://doi.org/10.1007/ S00726-009-0269-0
Yang J., Zhou Y., Jiang Y., 2022. Amino acids in rice grains and their regulation by polyamines and phytohormones. Plant, 11:1581. https://doi.org/10.3390/PLANTS 11121581
Yang Y., Zhu K., Xia H., Liang C., Keping C., 2014. Comparative proteomic analysis of indica and japonica rice varieties. Genet. Mol. Biol., 37 (4): 652–661. https://doi.org/10.1590/S1415-475720140 05000
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