Using radioisotope to study the dimethoate degradation capacity of bacteria

Abstract

The dimethoate (Di) biodegradation capacity of 2 bacterium strains Aerococcus sp.T and Neiseria sp.C isolated from agricultural soil of Dalat city was determined by using ³²P labeled dimethoate (³²P-Di). Adding ³²P-Di into the culture medium of these 2 bacterium strains, ³²P-Di was quickly biodegraded and at 72 hours of cultivation, ³²P-Di was not detected. After 120 hours of incubation, the radioactivity of ³²P was mostly accumulated in the medium, partly in the biomass of bacteria. Intermediates found in the biotransformation processes by the strains Aerococcus sp.T and Neiseria sp.C were less than compared to the non biotransformation process and the poisonous intermediates were not found.