Biosynthesis amylase and proteinase of some halophilic bacterial strain from shrimp culture ponds
Author affiliations
DOI:
https://doi.org/10.15625/0866-7160/v29n3.5389Abstract
By the screening sediements of shrimp culture pond, we selected two bacterial strains NB4 and CHP6, these bacterial strains could biosynthesize enzymes amylase and proteinase. They could grow and produced amylase and proteinase in the media with NaCl concentration ranged from 0% to 10%. The optimum sodium concentration of the media for their production of amylase was 5% NaCl and for their production of proteinase was 2% NaCL. These bacterial strains were halophilic microorganisms. These bacterial could grow and biosynthesize amylase and proteinase in the media with a range of incubation temperature from 20oC to 40oC, but most suitable temperature for their production of these enzymes were 30oC - 35oC. The optimum pH for biosyntheses amylase and proteinase were 6.5 - 8.5.
The highest biosynthesis of amylase occurred in media used starch as carbon source and beef extract as nitrogen source. The highest biosynthesis of proteinase in media contained glucose and soybean powder. The media containing organic nitrogen sources were suitable for the biosyntheses of mentioned above enzymes. The media containing inorganic nitrogen likes nitrogen source were very fragile for their production of amylase and proteinase. These strains could not grown and produced amylase and proteinase in the media containing ammonium sulfate as nitrogen source.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Academia Journal of Biology (AJB) is an open-access and peer-reviewed journal. The articles published in the AJB are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0), which permits for immediate free access to the articles to read, download, copy, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited (with a link to the formal publication through the relevant DOI), and without subscription charges or registration barriers. The full details of the CC BY-NC-ND 4.0 License are available at https://creativecommons.org/licenses/by-nc-nd/4.0/.