Point mutation analysis of the PBP2B (penicillin binding protein) gene in penicillin resistant Streptococcus pneumoniae strains
Author affiliations
DOI:
https://doi.org/10.15625/0866-7160/v25n1.6801Abstract
We amplified three fragments (2B1, 2B2, 2B3) corresponding to the transpeptidase encoding region of pbp2b gene in 20 penicillin-resistant strains of S. pneumoniae isolated from clinical samples. The fragments were then analysed by SSCP (Single Strand Conformation Polymorphism) electrophoresis.
We identified seven SSCP electrophoretic patterns of 2B1, four of 2B2 and five of 2B3 fragments. This high variation in electrophoretic mobility of these fragments shows the great genetic variation of the studied region. The sequencing of these fragments will confirm our results.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Academia Journal of Biology (AJB) is an open-access and peer-reviewed journal. The articles published in the AJB are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0), which permits for immediate free access to the articles to read, download, copy, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited (with a link to the formal publication through the relevant DOI), and without subscription charges or registration barriers. The full details of the CC BY-NC-ND 4.0 License are available at https://creativecommons.org/licenses/by-nc-nd/4.0/.
