Rapid micropropagation of Begonia tuberous by young leaf tissue culture and acclimatization in greenhouse
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DOI:
https://doi.org/10.15625/0866-7160/v26n3.6904Abstract
In this paper, the protocol for the rapid micropropagation of Begonia tuberous are presented.
The shoot regeneration was carried out on 1/2MS medium, plus 0.7 mg/l BA and 0.03 mg/l NAA. Furthermore, the callus formation was obtained on 1/2MS medium supplemented with 0.7 mg/l BA and 0.1 mg/l NAA.
For the shoot multiplication, single shoots were cultured on MS medium, plus 1 mg/l BA and 0.3 mg/l NAA.
The root formation was obtained on 1/2MS medium, plus 0.5 mg/l NAA and 0.3 mg/l IBA.
To increase the growth and development possibility of Begonia tuberous in the greenhouse, we can use some chemical fertilizers (nitrophoska, Grow more, super) at a suitable dosage.
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