Prevalence and reduction of Streptococcus mutans, Streptococcus sobrinus, and Streptococcus sanguinis in Vietnamese students following fluoride varnish application
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https://doi.org/10.15625/2615-9023/22600Keywords:
Bacterial prevalence, caries prevention, dental plaque, fluoride varnish, oral microbiome, pediatric dentistryAbstract
Dental caries is a multifactorial disease influenced by microbial composition, host-related factors, and environmental conditions. Streptococcus mutans and Streptococcus sobrinus are key contributors to caries progression, while Streptococcus sanguinis has a more complex role in caries development. This study aimed to determine the prevalence of S. mutans, S. sobrinus, and S. sanguinis in dental plaque among Vietnamese students and evaluate the antibacterial effects of fluoride varnish over a six-month period.
A cross-sectional analytical study was conducted at Vo Truong Toan Primary School, Can Tho City, Vietnam. Dental plaque samples were collected from 77 children with active caries and analyzed using PCR. Fluoride varnish (Clinpro™ White Varnish) was applied at baseline and three months post-treatment. Bacterial presence was re-evaluated at three and six months. The initial prevalence of S. mutans, S. sobrinus, and S. sanguinis was 50.6%, 14.3%, and 1.3%, respectively. After three months, S. mutans prevalence significantly declined to 19.5%, while S. sobrinus remained stable at 13.0%, and S. sanguinis increased slightly to 3.9%. At six months, S. mutans further decreased to 11.7%, and S. sobrinus to 6.5%, while S. sanguinis was detected in 2.6% of cases. The proportion of bacteria-free samples increased from 23.4% before treatment to 77.9% after six months. The findings confirm the dominant role of S. mutans in dental caries and highlight the sustained antibacterial effects of fluoride varnish in reducing its prevalence. S. sobrinus was detected less frequently but remained a significant contributor to caries, while S. sanguinis showed variable presence. These results support the use of fluoride varnish as a preventive measure for caries management. Future studies should explore bacterial load quantification, metagenomic profiling, and the long-term impact of fluoride varnish on microbial communities.
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References
Acevedo A. M., Ray M. V., Socorro M. & Rojas-Sanchez F., 2009. Frequency and distribution of Mutans Streptococci in dental plaque from caries-free and caries-affected Venezuelan children. Acta Odontol Latinoam, 22(1): 15−20. https://www.ncbi.nlm.nih.gov/pubmed/19601491
Ben Khadra G. M., Arrag E. A., Alammori M. & AlKadi M. F., 2019. The effect of chlorhexidine-thymol and fluoride varnishes on the levels of Streptococcus mutans in saliva in children aged 6–8 years. Indian J Dent Res, 30(1): 67−72. https://doi.org/10.4103/ijdr.IJDR_208_17
Coelho M. A. G., 2020. ICDAS and dmft/DMFT. Sensitivity and specificity, the importance of the index used: a systematic review. Journal of Dentistry & Public Health (inactive/archive only), 11(2): 176−187.
Conrads G., Flemmig T. F., Seyfarth I., Lampert F. & Lutticken R., 1999. Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR. J Clin Microbiol, 37(5): 1621−1624. https://doi.org/10.1128/JCM.37.5.1621-1624.1999
Erkmen Almaz M. & Akbay Oba A., 2020. Antibacterial activity of fluoride varnishes containing different agents in children with severe early childhood caries: a randomised controlled trial. Clin Oral Investig, 24(6): 2129−2136. https://doi.org/10.1007/ s00784-020-03300-w
Franco e Franco T. C., Amoroso P., Marin J. M. & de Avila F. A., 2007. Detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reaction. Braz Dent J, 18(4): 329−333. https://doi.org/10.1590/ s0103-64402007000400011
Le T. S., Nguyen P. T., Nguyen H. S. H., Nguyen T. P., Nguyen T. T., Thai M. N., Nguyen T. T. U., Nguyen M. C., Hoang Q. K. & Nguyen H. T., 2021. Expression of genes involved in exopolysaccharide synthesis in Lactiplantibacillus plantarum VAL6 under environmental stresses. Archives of Microbiology, 203(8): 4941−4950. https://doi.org/10.1007/ s00203-021-02479-0
Lemos J. A., Palmer S. R., Zeng L., Wen Z. T., Kajfasz J. K., Freires I. A., Abranches J. & Brady L. J., 2019. The Biology of Streptococcus mutans. Microbiol Spectr, 7(1). https://doi.org/10.1128/microbiol spec.GPP3-0051-2018
Luu H. L., Le D. V. & Phan T. P. T., 2018. Isolation of Streptococcus mutans causing dental caries and evaluation of its inhibition by some traditional medicinal herbs. Journal of Science and Technology Development: Natural Science, 2(2): 31−37.
Nguyen H. T., Nguyen K. T. K., Vo N. K. N., Lam H. L. & Thu H. A., 2023. Fabrication of nano berberine material and evaluation of its antibacterial activity against cariogenic bacteria. Can Tho University Journal of Science, 59(5): 25−31. https://doi.org/10.22144/ctujos.2023.183
Nguyen M. C., Le T. T. H. & Dao T. D., 2021. Effectiveness of caries prevention and treatment using 5% fluoride varnish and fluoride toothpaste in children aged 7−8 years. Vietnam Journal of Medicine, 505(1): 230−235. https://doi.org/ 10.51298/vmj.v505i1.1065
Nieminen A., Asikainen S., Torkko H., Kari K., Uitto V. J. & Saxen L., 1996. Value of some laboratory and clinical measurements in the treatment plan for advanced periodontitis. J Clin Periodontol, 23(6): 572−581. https://doi.org/10.1111/j.1600-051x.1996.tb01827.x
Okada M., Soda Y., Hayashi F., Doi T., Suzuki J., Miura K. & Kozai K., 2005. Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-school children. J Med Microbiol, 54(Pt 7): 661−665. https://doi.org/10.1099/jmm.0. 46069-0
Organization W. H., 2013. Oral Health Surveys: Basic Methods. World Health Organization. https://books.google.com.vn/books?id=8rEXDAAAQBAJ
Pandey S., Patnayak R. L., Khodiar P. K., Amle D., Pandey A. & Tripathi P., 2022. Molecular Detection of Streptococcus mutans and Streptococcus sobrinus in Dental Plaque Samples in Children Aged Six to Nine Years. Cureus, 14(12): e32672. https://doi.org/10.7759/cureus.32672
Paul S., Baranya Shrikrishna S., Suman E., Shenoy R. & Rao A., 2014. Effect of fluoride varnish and chlorhexidine-thymol varnish on Mutans Streptococci levels in human dental plaque: a double-blinded randomized controlled trial. Int J Paediatr Dent, 24(6): 399−408. https://doi.org/ 10.1111/ipd.12085
Pharmacy C. T. U. o. M. a., 2021. Pediatric Dentistry Textbook. Medical Publishing.
Piszko A., Piszko P. J., Lubojanski A., Grzebieluch W., Szymonowicz M. & Dobrzynski M., 2023. Brief Narrative Review on Commercial Dental Sealants-Comparison with Respect to Their Composition and Potential Modifications. Materials (Basel), 16(19): 6453. https://doi.org/10.3390/ma16196453
Setyarini W., Widjiastuti I., Ridwan R. D. & Kuntaman K., 2020. The distribution of Streptococcus mutans and Streptococcus sobrinusin children with dental caries severity level. Dental Journal Majalah Kedokteran Gigi, 53(1): 36−39. https://doi.org/10.20473/j.djmkg.v53.i1.p36-39
Singla D., Sharma A., Sachdev V. & Chopra R., 2016. Distribution of Streptococcus mutans and Streptococcus sobrinus in Dental Plaque of Indian Pre-School Children Using PCR and SB-20M Agar Medium. J Clin Diagn Res, 10(11): ZC60-ZC63. https://doi.org/10.7860/ JCDR/2016/19256.8909
Soyolmaa, M., Munguntsetseg, L., Sharkhuu, M.-O., Nishino, M., & Hulan, U., 2011. PCR detection of Streptococcus mutans and Streptococcus sobrinus in plaque samples from Mongolian mother-child pairs. Pediatric Dental Journal, 21(2): 154−159. https://doi.org/10.52547/rbmb. 12.1.205
Vu D. T., 2024. Prevalence, bacterial strains related to dental caries, and treatment outcomes using 38% silver diamine fluoride in 6-7-year-old students at Vo Thi Sau Primary School, Hai Duong City, PhD thesis, National Institute of Hygiene and Epidemiology, Hanoi.
Wang Y. X. & Liu X. J., 2013. The relationship between Streptococcus sobrinus and rampant caries in children. Shanghai Kou Qiang Yi Xue, 22(4): 432−437. https://www.ncbi.nlm.nih.gov/ pubmed/24100904
Yuan Q., Zhou C., Xie J., Zhang D., Zheng L., Li Y., Ren B., Peng X. & Zhou X., 2020. “Subgingival microbes,” in Atlas of oral microbiology: From healthy microflora to disease: 145−210, Springer.
Zhang K., Xiang Y., Peng Y., Tang F., Cao Y., Xing Z., Li Y., Liao X., Sun Y., He Y. & Ye Q., 2022. Influence of Fluoride-Resistant Streptococcus mutans Within Antagonistic Dual-Species Biofilms Under Fluoride In Vitro. Front Cell Infect Microbiol, 12: 801569. https://doi.org/ 10.3389/fcimb.2022.801569
Zhou X., 2016. Dental caries: principles and management. Springer.
Zubaidah N., Dianawati N., Ridwan R. D., Shirakawa T., Kuntaman K., Setiawatie E. M., Tanzil M. I. & Kunarti S., 2022. The clinical pattern and prevalence of Streptococcus mutans and Streptococcus sobrinus among adult and children patients with dental caries. Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 22: e210117.
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